甲型流感核酸RNA質(zhì)控品

廣州健侖生物科技有限公司

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甲型流感核酸RNA質(zhì)控品

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【公司名稱】 廣州健侖生物科技有限公司
【市場(chǎng)部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

5. 游離坐骨神經(jīng)和剪斷股骨：認(rèn)清坐骨神經(jīng)溝和腓腸肌的部位，用剪刀剪斷梨狀肌及其周圍的結(jié)締組織，左手提著神經(jīng)上的細(xì)線，右手持剪刀或玻璃分針沿坐骨神經(jīng)溝細(xì)心剝離，直至將坐骨神經(jīng)剝離到腘窩。將游離干凈的坐骨神經(jīng)放在下腿上，沿膝關(guān)節(jié)的周圍將大腿的所有肌腱剪斷，并用剪刀刮凈股骨下段附著的肌肉，在股骨上1/3處剪去上段股骨及所附的肌肉，這樣制成的稱為坐骨神經(jīng)下腿標(biāo)本。
6. 游離腓腸?。涸谧巧窠?jīng)下腿標(biāo)本的基礎(chǔ)上，用剪刀將跟腱的下端剪斷，在跟腱與肌肉交界處扎一條細(xì)線，左手提線，右手用剪刀游離腓腸肌，直到膝關(guān)節(jié)。zui后用粗剪刀在膝關(guān)節(jié)下將小腿剪去，留下的即為坐骨神經(jīng)腓腸肌標(biāo)本。做好的標(biāo)本用鋅銅叉的兩極輕輕接觸坐骨神經(jīng)，如腓腸肌立即收縮，表示標(biāo)本的興奮性良好，然后將標(biāo)本放入任氏液中，待其興奮性穩(wěn)定后再進(jìn)行實(shí)驗(yàn)。
注意事項(xiàng)
1. 已剝離皮膚的組織避免接觸皮膚毒液或其他不潔物。
2. 分離神經(jīng)時(shí)，一定要用玻璃分針，不能隨便用刀、剪進(jìn)行操作。
3. 不能過分牽拉神經(jīng)，以免造成損傷。
4. 標(biāo)本制備過程中應(yīng)適當(dāng)?shù)赜萌问弦簼駶?rùn)標(biāo)本。
5. 避免用手指或金屬器械接觸或夾持標(biāo)本的神經(jīng)肌肉部分。

5. Free sciatic nerve and cut the femur: recognize the parts of the sciatic nerve sulcus and gastrocnemius muscle, with the scissors to cut the piriformis and its surrounding connective tissue, the left hand carrying the nerve on the thin line, right hand holding scissors or glass minute hand along the sciatic nerve groove carefully peel , Until the sciatic nerve stripped to popliteal fossa. The free and clean sciatic nerve on the lower leg, along the knee around the thigh will be cut all the tendons, and use scissors to clean the muscles attached to the lower femur, the upper femur 1/3 cut off the upper femur and attached muscles, so Made of sciatic nerve lower leg specimens.
6. Free gastrocnemius: On the basis of sciatic nerve lower leg specimens, cut the lower end of the Achilles tendon with scissors, tie a thin line at the junction of Achilles tendon and muscle, raise the left hand and the right hand use scissors to free the gastrocnemius muscle until the knee joint. Finally, use the scissors to cut off the calf under the knee joint, leaving the sciatic nerve gastrocnemius specimen. Well done specimens with zinc copper fork gently touch the sciatic nerve poles, such as immediate contraction of the gastrocnemius, said specimens of excitability is good, and then the specimens into Ren's solution, to be excitatory stability after the experiment.
Precautions
1. Skin that has been stripped of skin Avoid contact with skin venom or other impurities.
2. Separation of nerves, we must use glass minutes, can not just use a knife, cut to operate.
3. Do not pull the nerves too much, so as not to cause damage.
4. Rinsate specimens should be properly wetted with specimens during specimen preparation.
5. Avoid touching or holding the neuromuscular parts of the specimen with your fingers or metal devices.