4種非典型肺炎聯(lián)合檢測(cè)試劑盒（PCR方法）

廣州健侖生物科技有限公司

One tube multiplex for detection of Mycoplasma pneumoniae, Chlamydia pneumoniae, Legionella pneumophila/ Legionella longbeachae and internal control
單管多重檢測(cè)肺炎支原體，肺炎衣原體，嗜肺軍團(tuán)桿菌，長(zhǎng)灘軍團(tuán)菌和內(nèi)控

4種非典型肺炎聯(lián)合檢測(cè)試劑盒（PCR方法）

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【公司名稱(chēng)】 廣州健侖生物科技有限公司
【市場(chǎng)部】    楊永漢

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5、間質(zhì)著色
著色部位主要在間質(zhì)，間質(zhì)著色的原因很多，如抗體與組織中的蛋白質(zhì)因蛋白疏水基團(tuán)相互作用形成非特異性的連接而著色，加一抗前的血清封閉這一步就是為了避免非特異型的結(jié)合。又如血清中的免疫球蛋白常常滲出到組織間質(zhì)，很容易與抗體結(jié)合，造成間質(zhì)著色，特別是lambda和kappa染色時(shí)。當(dāng)甲狀腺膠質(zhì)外溢到組織間質(zhì)時(shí)，做甲狀腺球蛋白染色也會(huì)出現(xiàn)間質(zhì)著色?？贵w不純或抗體被污染也可出現(xiàn)間質(zhì)著色，我們?cè)龅紺D20抗體不純，除了染上B細(xì)胞外還染上了間質(zhì)。
6、細(xì)胞漿著色
胞漿著色是所有“雜音”染色中有欺騙性的著色，著色區(qū)局限在細(xì)胞內(nèi)，間質(zhì)無(wú)著色，看上去與真實(shí)的免疫反應(yīng)著色幾乎一樣，很難區(qū)別。胞漿里含有較多的蛋白質(zhì)，因此，很多非特異性的染色除了見(jiàn)于間質(zhì)也可以出現(xiàn)在胞漿中。這種原因造成的著色，可以通過(guò)血清封閉解決。還有因內(nèi)源酶造成的著色，如血紅蛋白（紅細(xì)胞）、肌紅蛋白（肌細(xì)胞）、細(xì)胞色素（粒細(xì)胞、單核細(xì)胞）、過(guò)氧化氫酶（肝、腎），這些可用過(guò)氧化氫進(jìn)行封閉。巨噬細(xì)胞吞噬各種抗原物質(zhì)或Fc片斷而出現(xiàn)胞漿著色，這種著色不易避免，但可以通過(guò)形態(tài)學(xué)辨認(rèn)出巨噬細(xì)胞而引起重視。內(nèi)源性生物素的著色有欺騙性，因?yàn)樗鼜V泛的存在于組織細(xì)胞中，我們研究結(jié)果顯示：冰凍組織中存在內(nèi)源性生物素，經(jīng)福馬林固定石蠟包埋后生物素被封閉，加熱抗原修復(fù)后造成內(nèi)源性生物素暴露，內(nèi)源性生物素暴露的強(qiáng)度在不同的組織有所不同，從弱陽(yáng)性（+）到強(qiáng)陽(yáng)性（+++），內(nèi)源性生物素在組織中的分布形式，既有散在分布也有彌漫分布，主要以顆粒狀形式存在于胞漿中，內(nèi)源性生物素廣泛存在于上皮源性組織，特別是腺上皮組織，亦存在部分非上皮組織，內(nèi)源性生物素不僅存在人體組織也存在大鼠組織，內(nèi)源性生物素暴露的強(qiáng)弱與修復(fù)液有關(guān)，其強(qiáng)度增加依次為：檸檬酸、EDTA、EGTA，熱抗原修復(fù)暴露的內(nèi)源性生物素可被雞蛋清封閉，非生物素檢測(cè)系統(tǒng)Polymer兩步法（EliVision、EnVision）可避免生物素干擾。

5, interstitial coloring
Coloring sites mainly in the interstitial interstitial coloration for many reasons, such as antibodies and proteins in the tissue due to protein hydrophobic groups interact to form a non-specific connection and coloring, plus a pre-anti-serum before the step of this step is to avoid non-specific Type of combination. Another example is the serum immunoglobulin often exudes to the interstitial tissue, it is easy to combine with antibodies, causing interstitial staining, especially lambda and kappa staining. When the thyroid gland spill to the interstitial tissue, do thyroid globulin stain will appear interstitial staining. Antibody impure or antibody contamination can also occur interstitial staining, we have encountered CD20 antibody impure, in addition to B cells infected with the interstitial.
6, cytoplasm coloring
Cytoplasmic staining is the most deceptive coloring of any "murmur" staining. The pigmentation zone is confined within the cell with no staining in the stroma. It appears to be almost the same as the actual immune response, which is difficult to distinguish. Cytoplasm contains more protein, therefore, a lot of non-specific staining in addition to the stroma can also appear in the cytoplasm. Coloring caused by this reason can be solved by serum blocking. There are also endogenous enzymes caused by the coloring, such as hemoglobin (red blood cells), myoglobin (myocytes), cytochromes (granulocytes, monocytes), catalases (liver, kidney), these available hydrogen peroxide Closed. Macrophage phagocytosis of various antigenic material or Fc fragment cytoplasmic staining, this coloring is not easy to avoid, but macrophages can be identified by morphological attention. Pigmentation of endogenous biotin is the most deceptive because of its widespread presence in tissue cells. Our results show that endogenous biotin is present in the frozen tissue and that biotin is blocked after the paraffin-embedded paraffin-embedded , Heat antigen repair caused endogenous biotin exposure, endogenous biotin exposure intensity varies in different tissues, from weak positive (+) to strongly positive (+++), endogenous biotin in Tissue distribution, both scattered and diffuse distribution, mainly in granular form exists in the cytoplasm, endogenous biotin widespread in epithelial tissue, especially glandular epithelial tissue, there are also some non-epithelial tissue Endogenous biotin not only existed in human tissues but also existed in rat tissues. The strength of endogenous biotin exposure was related to repair fluid, and the intensities of endogenous biotin were as follows: citric acid, EDTA, EGTA, endogenous Biotin can be blocked with egg white, non-biotin detection system Polymer two-step method (EliVision, EnVision) to avoid biotin interference.